A COLORIMETRIC DETERMINATION OF INORGANIC PYROPHOSPHATE
نویسندگان
چکیده
منابع مشابه
A colorimetric determination of inorganic pyrophosphate.
During recent work on the mechanism of the ATP-CoA-acetate1 reaction (1, 2) we observed that, on incubation of yeast enzyme with ATP, CoA, acetate, and hydroxylamine, a substance was formed in the presence of fluoride which, although initially colorless with the Fiske-Subbarow phosphate reagents (3), slowly developed on standing a blue color of a somewhat more purplish shade than that obtained ...
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The formation of large amounts of inorganic pyrophosphate as a by-product of numerous synthetic reactions in nearly all living tissues makes the metabolic reactions of this compound of interest. Rapid hydrolysis to orthophosphate is presumed to be the fate of most inorganic pyrophosphate but the possibility of the conservation and utilization of some of the energy of this compound has been a su...
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A new and convenient method for the determination of P, was developed. Phosphomolybdate is measured calorimetrically, without reduction to molybdenum blue, by dissolving the whole assay mixture in acetone, where phosphomolybdate is bright yellow. The hydrolysis of acidlabile phosphates (e.g., creatine phosphate) causes no problems, because extra molybdate is complexed with citrate immediately a...
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Pyrophosphate anions play key roles in various biological and chemical processes. During the last few years, many exciting results have emerged regarding the development of fluorescent and colorimetric sensors for this biologically important species. In this review, we will cover the fluorescent and colorimetric chemosensors developed for the detection of pyrophosphate (PPi) since 2010.
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The methods most generally used for the determination of phosphates in blood are based upon the reduction of phosphomolybdic acid to the blue molybdic oxide, the color of which is compared to a solution containing a known amount of phosphate. The first method based upon this principle was introduced by Taylor and Miller (1) and was later modified by Bell and Doisy (2), Brig& (3), Benedict and T...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71166-6